ENZIM RESTRIKSI
Tabel 1. Beberapa enzim
restriksi dan urutan pemotongannya
|
NO
|
Mikroorganisme
|
Singkatan nama
enzim
|
Rangkaian Pemotongan
5’ 3’
3’ 5’
|
|
|
1
|
Anabaena
variabilitis
|
Ava1
|
G
(T) CG (G) G
G
AGCCC
|
|
|
2
|
Bacillus
amyloliquifaciens
H
|
Bam HI
|
G
GATCC
C
CTAGG
|
|
|
3
|
Bacillus
globigii
|
BglII
|
A
GATCT
T
CTAGA
|
|
|
4
|
Escherichia
coli RY
13
|
Eco RI
|
G
AATTC
CTTAAG
|
|
|
5
|
Haemophlius
aegybtius
|
Hae II
|
Pu
G C G C Py
Py
C G C G Pu
|
|
|
6
|
Haemophlius
gallinarum
|
Hga I
|
CGA
C GC
GCT
GCG
|
|
|
7
|
Haemophlius
haemolyticus
|
Hha I
|
GCG C
C
G CG
|
|
|
8
|
Haemophlius
influenzae
Rd
|
Hind I
Hind III
|
G
T (T) (G) A C
C
A (A) (C) T G
A
AGCTT
TTCGA
A
TTCGA
A
|
|
|
9
|
Haemophlius
parainflunzae
|
Hpa I
|
GTT
AAC
CAA
TT G
|
|
|
10
|
Klebsiella
pnewmoniae
|
Kpn III
|
GGTAC
C
CCATG
G
|
|
|
11
|
Moraxella
bovis
|
Mbo I
|
GATC
CTAG
|
|
|
12
|
Providencia
stuartii
|
Pst I
|
CTGCA
G
GACGT
C
|
|
|
13
|
Serratia
marcescens sb
|
Sma I
|
CCC
GGG
GGG CCC
|
|
|
14
|
Streptomyces
stanford
|
Sst I
|
GAGCT
C
CTCGA
G
|
|
|
15
|
Xanthomonas
malvacearum
|
Xma
|
C
CCGGG
G GGCCC
|
|
|
16
|
Streptomyces
albus G
|
SalI
|
G
TCGAC
C AGCTG
|
|
|
17
|
Xanthomonas
oryzae
|
XorII
|
CGATC
G
G
CTAGC
|
|
|
18
|
Brevibacterium
albidum
|
BalI
|
TGG
CCA
ACC
GGT
|
|
|
19
|
Escherichia coli R
245
|
Eco RII
|
CC TGG
GGT A C C
|
|
|
20
|
Haemophilus aegyptius
|
Hae III
|
GG CC
CC GG
|
|
|
21
|
Haemophilus aegyptius
|
Hpa II
|
C CGG
GGC C
|
|
|
22
|
Haemophilus influenzae Rd
|
Hind II
|
GTPy PuAC
CAPuPyT G
|
|
|
23
|
Atrobacter luteus
|
AlulI
|
AGCT
TCGA
|
|
|
24
|
Proteus vulgaris
|
PvulI
|
CAGCTG
GTCGAC
|
|
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